Occurrence of histones in the trypanosomatid flagellate Crithidia oncopelti

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Thermodynamic and Hydrodynamic Studies Relating to the Mechanochemical Cycle in the Flagellum of Crithidia Oncopelti

1. The motile response of the flagellum of Crithidia oncopelti to changes in the temperature, pressure and viscosity of the environment were studied. 2. The dependence of beat frequency on temperature and pressure can be interpreted in terms of the theory of reaction rates by making the assumption that the frequency is proportional to the rate constant of a chemical reaction within the flagellu...

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Introduction of plasmid DNA into the trypanosomatid protozoan Crithidia fasciculata.

Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resistant colonies were selected on agar plates containing paromomycin and screene...

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Effects of calcium on flagellar movement in the trypanosome Crithidia oncopelti.

1. The effects of calcium on the motility of different preparations of flagella from Crithidia oncopelti were studied using stroboscopic and high-speed cine photographic techniques. 2. By varying the concentration of calcium in suspensions of chemically treated samples of the organism it was found that changes occurred in bend shape, wave direction and frequency. 3. Waves on the flagellum of th...

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The effects of laser microbeam irradiation on the flagellum of Crithidia (Strigomonas) oncopelti.

The flagellum of the trypanosomatid flagellate Crithidia (Strigotnonas) oncopelti is capable of propagating waves from both its base and tip (Holwill, 1965). In the present study, the capabilities of bend initiation and propagation by the base, tip and points along the flagellum have been studied by observing the effects of irradiation of small regions with a pulsed ruby laser microbeam. The lo...

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Effects of pressure and temperature changes on the flagellar movement of crithidia oncopelti.

The fundamental processes which underly flagellar activity are molecular in nature and it is to be expected that environmental changes which affect chemical processes will also modify the movement of flagella. In particular, the equilibrium state and the rate of a chemical reaction depend on the hydrostatic pressure and temperature to which the system is exposed, and it is possible to derive ch...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1971

ISSN: 0306-3283

DOI: 10.1042/bj1250044pa